Microcystin-LR, a potent protein phosphatase inhibitor, prolongs the serotonin- and cAMP-induced currents in sensory neurons of Aplysia californica.
نویسندگان
چکیده
Microcystin-LR (MCYST-LR), a hepatotoxin produced by cyanobacteria, inhibited purified protein phosphatases (PrPs) from rabbit skeletal muscle and the enzymes from Aplysia with an IC50 of approximately 10(-10) M. MCYST-LR also prolonged both serotonin- (5-HT) and cyclic adenosine monophosphate-induced inward currents in sensory neurons of Aplysia. These results, which are consistent with inhibition of Aplysia PrPs, indicate that MCYST-LR may be a useful probe to elucidate the function of PrPs in neural tissues.
منابع مشابه
Role of protein phosphatases in the modulation of neuronal membrane currents.
Although much is known regarding the modulation of ion channels through protein phosphorylation, little is known about their dephosphorylation by protein phosphatases (PrPs). To address this issue we examined the effects of a phosphatase inhibitor, okadaic acid (OA), and protein phosphatases obtained from rabbit skeletal muscle on steady-state membrane current in voltage-clamped sensory neurons...
متن کاملModulation of a transient K+ current in the pleural sensory neurons of Aplysia by serotonin and cAMP: implications for spike broadening.
To study the contribution of cAMP to the spike broadening produced by serotonin (5-HT) in the pleural sensory neurons of the tail withdrawal reflex, we utilized two phosphodiesterase-resistant cAMP analogs: the Sp diastereomer of cyclic adenosine 3',5'-monophosphothioate (Sp-cAMP[S]), which activates protein kinase A, and the antagonist Rp diastereomer of cyclic adenosine 3',5'-monophosphothioa...
متن کاملAge-related deficits in synaptic plasticity rescued by activating PKA or PKC in sensory neurons of Aplysia californica
Brain aging is associated with declines in synaptic function that contribute to memory loss, including reduced postsynaptic response to neurotransmitters and decreased neuronal excitability. To understand how aging affects memory in a simple neural circuit, we studied neuronal proxies of memory for sensitization in mature vs. advanced age Aplysia californica (Aplysia). L-Glutamate- (L-Glu-) evo...
متن کاملSerotonin activates S6 kinase in a rapamycin-sensitive manner in Aplysia synaptosomes.
The identification of tags that can specifically mark activated synapses is important for understanding how long-term synaptic changes can be restricted to specific synapses. The maintenance of synapse-specific facilitation in Aplysia sensory to motor neuron cultures can be blocked by inhibitors of translation and by the drug rapamycin, which specifically blocks a signaling pathway that regulat...
متن کاملSensitizing stimuli cause translocation of protein kinase C in Aplysia sensory neurons.
The defensive tail-withdrawal reflex of Aplysia californica, mediated by identified sensory neurons in pleural ganglia that form synapses on motor cells in pedal ganglia, can be sensitized by stimulating the animal with electric shock. The neurophysiological basis of this simple form of learning is thought to be the increased release of transmitter by the sensory neurons. Earlier work has focus...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Brain research
دوره 533 1 شماره
صفحات -
تاریخ انتشار 1990